Free Practice Test: AP Biology

Last updated: August 16, 2026

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AP exams let you earn college credit, test out of an intro-level class, or both, while showing college admissions officers you can handle rigorous coursework. Most colleges and universities in the US recognize AP credit for a strong score.
AP Biology covers roughly what you'd see in a two-semester college introductory biology sequence, from molecules to ecosystems. It's a content-heavy course, but the exam itself is built around a smaller set of recurring "big ideas" (evolution, energy processing, information storage and transmission, and system interactions) that show up again and again no matter which unit a question is drawn from.

Fast Biology Study Guide

The AP Biology exam runs about 3 hours. Section I is 60 multiple-choice questions in 90 minutes, including several sets built around a shared data table, graph, or experimental scenario. Section II is six free-response questions in 90 minutes: two long-form questions (one involving interpretation of provided data or a model, one involving experimental design or analysis) and four short-answer questions, one of which requires you to read a brief text passage. Multiple-choice and free-response sections are weighted equally toward your final score of 1 to 5. Because this is a quantitative, data-heavy exam, you're allowed an approved four-function, scientific, or graphing calculator for the entire test. Double-check exact timing and calculator policy against the current AP Course and Exam Description before test day.

Chemistry of Life (8–11%)

This unit is the chemistry prerequisite for everything else in the course: water's properties (polarity, hydrogen bonding, cohesion, and why that matters biologically), pH and buffers, and the structure and function of the four major macromolecules (carbohydrates, lipids, proteins, and nucleic acids). You'll need to recognize monomer/polymer relationships (like amino acids building proteins) and understand dehydration synthesis and hydrolysis as the reactions that build and break these molecules.

Cell Structure, Function, and Communication (10–13%)

Covers the structure of prokaryotic and eukaryotic cells, organelle function, and the plasma membrane's role in regulating what enters and exits the cell: passive transport (diffusion, osmosis, facilitated diffusion) versus active transport. Cell communication (signal transduction pathways) and the cell cycle, including mitosis and its checkpoints, live here too. Know what happens when checkpoint regulation fails, since that's the biological basis of several exam questions on cancer.

Cellular Energetics (12–16%)

This unit tests photosynthesis and cellular respiration in real depth: the light-dependent reactions and Calvin cycle for photosynthesis, and glycolysis, the Krebs cycle, and oxidative phosphorylation for respiration. Enzyme structure and function (active sites, substrate specificity, and how temperature, pH, and inhibitors affect enzyme activity) is also tested heavily, often through a graph you have to interpret rather than a definition you have to recall.

Heredity and Gene Expression (25–33% combined)

Together the largest chunk of the exam. Heredity covers Mendelian genetics (monohybrid and dihybrid crosses, Punnett squares), non-Mendelian inheritance patterns (incomplete dominance, codominance, sex-linked traits), and meiosis as the mechanism that generates genetic variation. Gene expression covers DNA replication, transcription, and translation in detail, plus gene regulation (operons in prokaryotes, and epigenetic and transcription factor control in eukaryotes) and biotechnology tools like PCR and gel electrophoresis.

Natural Selection (13–20%)

Evolution is one of the College Board's designated "big ideas," and it shows up throughout the exam, not just in this unit. Know the core evidence for evolution (fossil record, comparative anatomy and embryology, molecular biology), the Hardy-Weinberg equilibrium equation for population genetics, and mechanisms of evolutionary change (natural selection, genetic drift, gene flow, and mutation). Speciation (how new species form through reproductive isolation) and phylogenetic trees, which you'll be asked to read and construct, round out the unit.

Ecology (10–15%)

The final unit zooms out to populations, communities, and ecosystems. You'll need population growth models (exponential vs. logistic growth, carrying capacity), community interactions (competition, predation, symbiosis), energy flow through trophic levels and food webs, and nutrient cycling (carbon, nitrogen, water). Expect at least one free-response question that asks you to interpret a population or energy-flow graph rather than simply recall a definition.

Biology Free Practice Test

So, are you ready to test the waters? Take this practice quiz and judge your preparation level before diving into deeper study. AP test questions cover a mix of multiple-choice questions and free-response questions. The following are original sample questions, written by Powerhouse Prep, in the style of the types of questions that may appear on the exam.

Question 1: A cell is placed in a solution with a lower solute concentration than the cell's cytoplasm. What will happen to the cell?

  1. Water will move out of the cell, causing it to shrink
  2. Water will move into the cell, causing it to swell or burst
  3. No net movement of water will occur
  4. Solutes will move into the cell to equalize concentration

Correct Answer: B. Water will move into the cell, causing it to swell or burst

Explanation: A solution with a lower solute concentration than the cell is hypotonic relative to the cell. Water moves by osmosis from an area of lower solute concentration (higher water concentration) to an area of higher solute concentration, so water flows into the cell, causing it to swell (and potentially lyse, if it's an animal cell without a rigid cell wall to resist the pressure).


Question 2: In a dihybrid cross between two heterozygous pea plants (YyRr x YyRr), what fraction of offspring is expected to be homozygous recessive for both traits (yyrr), assuming independent assortment?

  1. 1/4
  2. 1/8
  3. 1/16
  4. 9/16

Correct Answer: C. 1/16

Explanation: For each gene individually, a heterozygous x heterozygous cross (Yy x Yy) produces homozygous recessive offspring (yy) at a rate of 1/4. Since the two genes assort independently, you multiply the probabilities: 1/4 (yy) x 1/4 (rr) = 1/16. This is the same logic behind the classic 9:3:3:1 phenotypic ratio in a dihybrid cross, where the double-recessive phenotype makes up 1 of the 16 total parts.


Question 3: During cellular respiration, most of the ATP produced comes from which stage?

  1. Glycolysis
  2. Pyruvate oxidation
  3. The Krebs (citric acid) cycle
  4. Oxidative phosphorylation (the electron transport chain and chemiosmosis)

Correct Answer: D. Oxidative phosphorylation (the electron transport chain and chemiosmosis)

Explanation: While glycolysis and the Krebs cycle each produce a small amount of ATP directly (through substrate-level phosphorylation), the vast majority of ATP is generated during oxidative phosphorylation, when NADH and FADH2 deliver electrons to the electron transport chain, which pumps protons across the mitochondrial membrane to drive ATP synthase via chemiosmosis.


Question 4: A population of beetles has two color morphs: green and brown. Birds prey heavily on the green beetles because they stand out against tree bark, while brown beetles are camouflaged. Over many generations, the population becomes almost entirely brown. This is an example of which type of natural selection?

  1. Disruptive selection
  2. Directional selection
  3. Stabilizing selection
  4. Sexual selection

Correct Answer: B. Directional selection

Explanation: Directional selection favors one extreme phenotype (brown) over the other (green), shifting the population's trait distribution in one direction over time. Disruptive selection favors both extremes over the middle; stabilizing selection favors the middle phenotype over both extremes. Since there's no evidence of mate choice driving the color difference, this isn't sexual selection. It's predation pressure, a form of natural selection.


Question 5: Which of the following best explains why an operon like the lac operon is efficient for a bacterial cell?

  1. It allows genes to be transcribed continuously regardless of environmental conditions
  2. It groups genes with related functions under a single promoter, so they are transcribed together only when needed
  3. It prevents any transcription from occurring in the cell
  4. It converts RNA directly into protein without translation

Correct Answer: B. It groups genes with related functions under a single promoter, so they are transcribed together only when needed

Explanation: An operon clusters genes that work together (like the genes needed to metabolize lactose) under one promoter and one regulatory switch, so the cell can turn all of them on or off together in response to a single environmental cue: in the lac operon's case, the presence or absence of lactose. That's more efficient than regulating each gene individually, and it avoids wasting energy transcribing genes the cell doesn't currently need.


More AP Biology Study Resources

Looking for a study guide to fill a couple gaps, or just want a full length practice exam? A few resources worth checking out below. If you do find these recommendations useful, please shop through the affiliate links to help support this site.

Barron's AP Biology Premium Study Guide

A thorough unit-by-unit content review with practice tests, well matched to the exam's emphasis on data interpretation.


Princeton Review Cracking the AP Biology Exam

Strong on free-response strategy and pacing, which matters given how time-pressured the six-question FRQ section can feel.


AP Biology Flashcards

Useful for drilling vocabulary-heavy topics like enzyme kinetics, cell signaling pathways, and the steps of protein synthesis.


Plenty of other resources exist – just do a quick internet search – but these are a good place to start.